AKR1C2 蛋白, Human (His)

AKR1C2 protein: Cytoplasmic aldehyde-keto reductase, which uses NADH and NADPH to reduce ketosteroids to hydroxysteroids. AKR1C2 Protein, Human (His) is the recombinant human-derived AKR1C2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of AKR1C2 Protein, Human (His) is 323 a.a., with molecular weight of ~38 kDa.

AKR1C2 蛋白是一种胞质醛酮还原酶，在催化 NADH 和 NADPH 依赖性酮类固醇还原为羟基类固醇方面发挥着关键作用。这种酶在体内表现出可能的还原酶功能，正如生理浓度的 NADPH 在体外抑制其氧化酶活性所证明的那样。AKR1C2 显示出广泛的位置特异性，作用于类固醇的 3、17 和 20 位，从而调节雌激素和雄激素等激素的代谢。它与 5-α/5-β-类固醇还原酶合作，有助于将类固醇激素转化为 3-α/5-α 和 3-α/5-β-四氢类固醇。此外，AKR1C2 催化强效雄激素 5-α-二氢睾酮 (5-α-DHT) 失活为 5-α-雄甾烷-3-α,17-β-二醇 (3-α-二醇)。具体来说，它能够产生 17β-羟基-5α-雄激素-3-酮/5αDHT，并且还可以减少缀合类固醇，例如硫酸 5α-二氢睾酮。此外，该蛋白质表现出对胆汁酸的亲和力，进一步强调了它参与多种代谢途径。

Measured by its ability to oxidize S-1-indanol in the presence of NADP+ that incubate at roomtemperature in kinetic mode for 5 minutes. The specific activity is 54.55 pmol/min/µg.

Human

E. coli

N-6*His

P52895 (M1-Y323)

1646  [NCBI]

MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEAVKLAIEAGFHHIDSAHVYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSNSHRPELVRPALERSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEAMEKCKDAGLAKSIGVSNFNHRLLEMILNKPGLKYKPVCNQVECHPYFNQRKLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAIDGLNRNVRYLTLDIFAGPPNYPFSDEY

Approximately 38 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.