ALCAM/CD166 蛋白, Cynomolgus (HEK293, His)

ALCAM/CD166 Protein is a glycoprotein involved in homotypic and heterotypic cell adhesion. ALCAM/CD166 has emerged as a modulator of cancer progression, mainly by controlling cell proliferation, adhesion, migration and invasion^[1]. ALCAM/CD166 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag. The total length of ALCAM/CD166 Protein, Cynomolgus (HEK293, His) is 499 a.a., with molecular weight of ~75-95 kDa.

活化白细胞细胞粘附分子 (ALCAM) 或分化簇 166 (CD166) 是一种与免疫球蛋白超家族分子结构相关的细胞表面糖蛋白，最初是在活化白细胞上发现的。ALCAM 还存在于上皮细胞、血管内皮细胞、神经元、骨髓祖细胞、造血干细胞、间充质干细胞、骨髓基质细胞、肿瘤细胞和癌症干细胞中。ALCAM 可以在细胞表面被金属蛋白酶进行蛋白水解切割，从而产生其胞外域的脱落。在正常生理学中，ALCAM 已被证明可以调节细胞与淋巴细胞以及上皮细胞和内皮细胞之间的相互作用。除了细胞粘附之外，ALCAM 的其他功能还包括血管生成、单核细胞迁移、白细胞跨血脑屏障内渗、T 细胞激活、造血、神经突延伸、成骨和子宫内胚胎植入^[1]。

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human CD6. The ED₅₀ for this effect is 102.3 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human CD6. The ED₅₀ for this effect is 102.3 ng/mL.

Cynomolgus

HEK293

C-His

G7NZQ8 (W28-A526)

/

WYTVNSAYGDTIIMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTVVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTVHSEQAVFDIYYPTEQVTIQVLPPKTAIKEGDNVTLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQA

Approximately 75-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.