ALCAM/CD166 蛋白, Rat (HEK293, His)

ALCAM/CD166 protein is a multifunctional cell adhesion molecule involved in various cell-cell interactions. It promotes T cell activation and immune synapse formation by interacting with CD6. ALCAM/CD166 Protein, Rat (HEK293, His) is the recombinant rat-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag. The total length of ALCAM/CD166 Protein, Rat (HEK293, His) is 500 a.a., with molecular weight of 68-95 kDa.

ALCAM/CD166 蛋白是一种细胞粘附分子，在细胞间相互作用中发挥多种作用。它通过与 CD6 的相互作用介导异型接触和同型接触。它与 CD6 的相互作用促进 T 细胞活化、增殖和免疫突触的形成。ALCAM 还介导与表达 ALCAM 的细胞的同型相互作用，并促进树突细胞与内皮细胞的附着。此外，它还抑制内皮细胞迁移，促进内皮管形成，并且对于淋巴管网络的正常组织是必需的。ALCAM 还参与造血，因为它是骨髓中正常数量的造血干细胞和造血干细胞植入所必需的。此外，它还促进成骨细胞增殖和分化，以及神经突延伸、轴突生长和轴突引导。ALCAM 促进视网膜神经节细胞轴突的生长和寻路。它形成同型二聚体并与 CD6 相互作用，两种相互作用都发生在细胞外结构域中。然而，同二聚化和CD6相互作用不能同时发生，并且对CD6的亲和力高于自缔合。最后，ALCAM 可以与 LGALS1 和 LGALS3 相互作用，但这种相互作用会抑制其与 CD6 的相互作用，并涉及糖基化的胞外结构域。

Measured by its ability to block adhesion of HuT78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6. Recombinant Rat ALCAM/CD166 at 10 µg/mL blocked 81.21% of HUT78 cell adhesionto immobilized recombinant human CD6 (10 µg/mL, 100 µL/well).

Rat

HEK293

C-His

O35112 (W28-K527)

79559  [NCBI]

WYTVNSAYGDTIVMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSINNAKISDEKRFVCMLVTEDNVFEAPTLVKVFKQPSKPEIVNRAAFLETEQLKKLGDCISRDSYPDGNITWYRNGKVLQPVDGEVSILFKKEIDPGTQLYTMTSSLEYKTTKSDIQMPFTCSVTYYGPSGQKTIYSEQAIFDIYYPTEQVTIQVLPPKNAIKEGDNITLQCLGNGNPPPEEFMFYLPGQAEGIRSSNTYTLTDVRRNATGDYKCSLIDQRNMAASTTITVHYLDLSLNPSGEVTKQIGDTLPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADDISDENREKVNDQAK

68-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.