ALDH1A1 蛋白, Human (His)

ALDH1A1 Protein, Human (His) is a human recombinant ALDH1A1 with an N-terminal Met and His tag produced in E. coli. ALDH1A1 Protein, Human (His) contains 501 amino acids (1-501 a.a.).

乙醛脱氢酶 1-A1 (ALDH1A1) 是乙醛脱氢酶家族的一部分。人 ALDH1A1 是一种胞质同源四聚体，在多种组织（皮肤、眼睛、肝脏、肾脏、睾丸和大脑）中表达。ALDH1A1 可氧化多种脂肪族和芳香族醛，包括内源性形成的醛类，例如视黄醛和 3,4-二羟基苯乙醛 (DOPAL)、异生物质（例如苯甲醛）或脂质过氧化过程中产生的醛类^[1]。

Measured by its ability to produce NADH during the oxidation of propionaldehyde. The specific activity is 77.63 pmol/min/µg, as measured under the described condition.

Human

E. coli

N-6*His

P00352 (M1-S501)

216  [NCBI]

MSSSGTPDLPVLLTDLKIQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIPIDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQYDKILDLIESGKKEGAKLECGGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGNGRELGEYGFHEYTEVKTVTVKISQKNS

Approximately 57 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 20% Glycerol, 1 mM DTT, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 20% Glycerol, 1 mM Dithiothreitol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Calleja LF, et al. Omeprazole as a potent activator of human cytosolic aldehyde dehydrogenase ALDH1A1. Biochim Biophys Acta Gen Subj. 2020 Jan;1864(1):129451.  [Content Brief]