1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. ALDH3A1 蛋白, Human (HEK293, His)

ALDH3A1 蛋白, Human (HEK293, His)

目录号: HY-P7477
产品使用指南

ALDH3A1 蛋白, Human (HEK293, His)是在 N 端带有 His 标签的人重组 ALDH3A1,在 HEK293 细胞中表达。

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描述

ALDH3A1 Protein, Human (HEK293, His) is a human recombinant ALDH3A1 with a His tag at the N-terminus. ALDH3A1 Protein, Human (HEK293, His) is expressed in HEK293 cells.

研究背景

醛脱氢酶 3A1 (ALDH3A1) 是 ALDH 超家族的重要成员,该家族由 NAD(P)+ 依赖性酶组成,可将多种内源产生和外源衍生的醛氧化为其相应的羧酸。ALDH3A1 通常在食管粘膜、小唾液腺、鼻上皮、扁桃体和口腔上皮中过表达。ALDH3A1 已被证明在多种癌症类型中表达上调,例如胃癌、肺癌或口腔鳞状细胞癌 (OSCC)[1]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P30838 (M1-Q452)

基因 ID

218  [NCBI]

蛋白结构
N-term
ALDH3A1 (M1-Q452)
Accession # P30838
6*His
C-term
中文名
重组人类醛脱氢酶家族 3 成员 A1,His (HEK293 细胞表达)
同用名
rHuAldehyde dehydrogenase family 3 member A1, His; ALDH-3A1; Aldehyde Dehydrogenase 3 member A1
氨基酸序列

MSKISEAVKRARAAFSSGRTRPLQFRIQQLEALQRLIQEQEQELVGALAADLHKNEWNAYYEEVVYVLEEIEYMIQKLPEWAADEPVEKTPQTQQDELYIHSEPLGVVLVIGTWNYPFNLTIQPMVGAIAAGNSVVLKPSELSENMASLLATIIPQYLDKDLYPVINGGVPETTELLKERFDHILYTGSTGVGKIIMTAAAKHLTPVTLELGGKSPCYVDKNCDLDVACRRIAWGKFMNSGQTCVAPDYILCDPSIQNQIVEKLKKSLKEFYGEDAKKSRDYGRIISARHFQRVMGLIEGQKVAYGGTGDAATRYIAPTILTDVDPQSPVMQEEIFGPVLPIVCVRSLEEAIQFINQREKPLALYMFSSNDKVIKKMIAETSSGGVAANDVIVHITLHSLPFGGVGNSGMGSYHGKKSFETFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQHHHHHHH

分子量

Approximately 58.0 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

ALDH3A1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ALDH3A1 Protein, Human (HEK293, His)
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