Aldolase B 蛋白, Human (N-GST)

Aldolase B protein catalyzes fructose 1,6-bisphosphate to trisaccharide phosphate during glycolysis and performs reverse aldol addition during gluconeogenesis. Aldolase B Protein, Human (N-GST) is the recombinant human-derived Aldolase B protein, expressed by E. coli , with N-GST labeled tag. The total length of Aldolase B Protein, Human (N-GST) is 363 a.a., with molecular weight of ~60 kDa.

醛缩酶 B 蛋白通过催化果糖 1,6-二磷酸羟醛裂解为磷酸二羟丙酮和 D-甘油醛 3-磷酸，以及糖异生中的反向立体特异性羟醛加成反应，在糖酵解中发挥关键作用。此外，在果糖分解过程中，它将膳食果糖通过果糖激酶磷酸化而产生的 1-磷酸果糖代谢为磷酸二羟丙酮和 D-甘油醛。除了其酶促功能外，醛缩酶 B 还充当接头，并通过稳定与 G6PD 和 TP53 的三元复合物来发挥肿瘤抑制因子的作用，从而抑制 G6PD 活性。这种调节机制有助于维持对氧化戊糖磷酸代谢的控制，有助于细胞代谢途径的整体平衡。

Measured by its ability to catalyzes the decomposition of fructose 1,6 bisphosphate in the presence of NADH. The specific activity is 4.1 U/mg.

Human

E. coli

N-GST

P05062 (A2-Y364)

229

AHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY

Approximately 60 KDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.