Alkaline Phosphatase/ALPP 蛋白, Cynomolgus (HEK293, His)

Alkaline Phosphatase (ALPP) is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. Zinc and magnesium are essential cofactors of ALPP. ALPP down-regulates the secretory activities of the intrahepatic biliary epithelium^[1][2][3]. Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Alkaline Phosphatase/ALPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is 483 a.a., with molecular weight of 57-67 kDa.

碱性磷酸酶 (ALPP) 是一组同工酶，通常位于细胞膜的外层。ALPP 催化细胞外空间中发现的有机磷酸酯的水解。ALPP 是一种普遍存在的膜结合糖蛋白，可在碱性 pH 值下催化磷酸单酯的水解^[1]。锌和镁是 ALPP 的重要辅助因子。此外，ALPP 还可下调肝内胆管上皮的分泌活性^[3]。
ALPP 可分为两种类型：组织特异性 ALPP 和组织非特异性 ALPP。组织特异性 ALPP 只存在于肠道、胎盘和生发组织中。在特定条件下，组织特异性 ALP 也可能有助于血清 ALP 的循环池。血清中 80% 以上的 ALP 来源于肝脏和骨骼^[2]。

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase, His Tag with an affinity constant of 79.52 nM as determined in SPR assay (Biacore T200).

Cynomolgus

HEK293

C-His

XP_045223825.1 (I21-T503)

102133045  [NCBI]

57-67 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 25mM Tris, 100mM NaCl, 1mM MgCl2, pH 7.5.Normally 8%

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.