1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. ALOX12 蛋白, Human (His)

ALOX12 蛋白, Human (His)

目录号: HY-P72076
COA 产品使用指南

ALOX12 蛋白, Human (His) 是一种带有 His 标签的重组 ALOX12 蛋白。ALOX12 编码花生四烯酸 12 脂氧合酶,作用于不同的多不饱和脂肪酸底物,产生生物活性脂质介质,包括二十烷和脂氧合酶。

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10 μg ¥2650 In-stock
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MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

ALOX12 Protein, Human (His) is a recombinant ALOX12 protein with a His tag. ALOX12 encodes arachidonic acid 12-lipoxygenase that acts on different polyunsaturated fatty acid substrates to produce biologically active lipid mediators including eicosanes and lipoxins[1].

研究背景

ALOX12主要将花生四烯酸代谢为12S-氢过氧二十碳四烯酸(12(S)-HpETE),并进一步通过过氧化谷胱甘肽将其还原为12(S)-HETE。ALOX12 广泛表达于多种细胞类型中。由于ALOX12具有调节血小板聚集、细胞迁移和肿瘤细胞增殖的功能,因此主要参与血栓形成、动脉粥样硬化、癌症等疾病的发生发展。ALOX12 参与炎症和细胞凋亡的调节,并通过激活 NF-kB 信号通路和 Caspase-3 参与肝损伤的发生。ALOX12在疾病的发生、发展中发挥着重要作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P18054 (M1-I663)

基因 ID

239  [NCBI]

蛋白结构
N-term
6*His
ALOX12 (M1-I663)
Accession # P18054
C-term
中文名
ALOX12 蛋白,人 (His 标签)
同用名
12 LOX; 12S; lipoxygenase ; 12-lipoxygenase; 12LO; 12S LOX; 12S-lipoxygenase; 12S-LOX; 12S-type; Alox12; Arachidonate 12 lipoxygenase; Arachidonate 12 lipoxygenase, 12S type; Arachidonate 12-lipoxygenase; Arachidonate 12-oxidoreductase; Lipoxin synthase 12-LO; LOG12; LOX12_HUMAN; P-12LO; Platelet 12-LOX; Platelet type lipoxygenase 12 ; platelet-type 12-lipoxygenase; Platelet-type lipoxygenase 12
氨基酸序列

MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI

分子量

Approximately 79.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献

ALOX12 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ALOX12 Protein, Human (His)
目录号:
HY-P72076
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