2. 重组蛋白
  3. Enzymes & Regulators
  4. Protease Inhibitors
  5. Serpin (Protease Inhibitor)
  6. Alpha-1 Antitrypsin 1-1
  7. Serpin A1a 蛋白, Mouse (HEK293, His)

Serpin A1a 蛋白, Mouse (HEK293, His)

目录号: HY-P7491
COA 产品使用指南

Alpha-1 protease inhibitor 1 蛋白, Mouse (HEK293, His)是在 C 末端带有 His 标签的小鼠重组 Serpin A1a，由哺乳动物表达系统产生。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μg ￥1700 In-stock
50 μg ￥4800 In-stock
100 μg   询价  

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描述

Alpha-1 protease inhibitor 1 Protein, Mouse (HEK293, His) is a mouse recombinant Serpin A1a with a His tag at the C-terminus. Alpha-1 protease inhibitor 1 Protein, Mouse (HEK293, His) is produced by Mammalian expression system.
研究背景

Alpha-1-抗胰蛋白酶 1-1（Serpin A1a；AAT）是最丰富的循环丝氨酸蛋白酶抑制剂（serpin），也是丝氨酸蛋白酶抑制剂超家族的原型成员。52 kD Serpin A1a 蛋白由基因 SERPINA1 编码。Serpin A1a 在调节免疫、炎症、蛋白质稳态、细胞凋亡以及可能的细胞衰老程序中发挥着重要作用。由于基因突变而导致的 Serpin A1a 系统性缺陷可导致肝衰竭和肺气肿等慢性肺部疾病[1]。
种属

Mouse
表达系统

HEK293
标签

C-6*His
蛋白编号

Q00898 (E25-K413)
基因 ID
蛋白结构
N-term
Serpin A1a (E25-K413)
Accession # Q00898
6*His
C-term
中文名
重组小鼠 Alpha-1-抗胰蛋白酶 1-1，His (HEK293 细胞表达)
同用名
rMuAlpha-1-antitrypsin 1-1, His; Serpin A1a; Alpha-1-antitrypsin 1-1
氨基酸序列

EDVQETDTSQKDQSPASHEIATNLGDFAISLYRELVHQSNTSNIFFSPVSIATAFAMLSLGSKGDTHTQILEGLQFNLTQTSEADIHKSFQHLLQTLNRPDSELQLSTGNGLFVNNDLKLVEKFLEEAKNHYQAEVFSVNFAESEEAKKVINDFVEKGTQGKIAEAVKKLDQDTVFALANYILFKGKWKKPFDPENTEEAEFHVDESTTVKVPMMTLSGMLHVHHCSTLSSWVLLMDYAGNATAVFLLPDDGKMQHLEQTLSKELISKFLLNRRRRLAQIHFPRLSISGEYNLKTLMSPLGITRIFNNGADLSGITEENAPLKLSQAVHKAVLTIDETGTEAAAVTVLQMVPMSMPPILRFDHPFLFIIFEEHTQSPIFLGKVVDPTHKHHHHHH

分子量

52-65 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filter solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)
参考文献

Serpin A1a Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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