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  3. Alpha-Fetoprotein 蛋白, Mouse (HEK293, His)

Alpha-Fetoprotein 蛋白, Mouse (HEK293, His)

目录号: HY-P7464
COA 产品使用指南

Alpha-FetoProtein, Mouse (HEK293, His)是在 C 末端带有 His 标签的小鼠重组 AFP,由哺乳动物表达系统产生,并表达编码 Lys19-Val605 的靶基因。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥530 In-stock
10 μg ¥890 In-stock
50 μg ¥2500 In-stock
100 μg ¥4250 In-stock
> 100 μg   询价  

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描述

Alpha-FetoProtein, Mouse (HEK293, His) is a mouse recombinant AFP with a His tag at the C-terminus. Alpha-FetoProtein, Mouse (HEK293, His) is produced by Mammalian expression system and the target gene encoding Lys19-Val605 is expressed.

研究背景

甲胎蛋白(AFP)是一种血清糖蛋白,通过受体介导的内吞作用进入胚胎细胞。AFP 是病理状况的标志物,包括肿瘤和非肿瘤疾病、抗氧化作用、不同细胞和癌症中的生长调节剂、免疫反应调节剂以及脂肪酸和雌激素的载体。AFP 传递的毒素会损害骨髓源性抑制细胞,从而释放先天性和适应性免疫来消灭癌细胞[1][2]

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P02772 (K19-V605)

基因 ID
蛋白结构
N-term
Alpha-Fetoprotein (K19-V605)
Accession # P02772
His
C-term
中文名
重组小鼠甲胎蛋白,His (HEK293 细胞表达)
同用名
rMuAlpha-Fetoprotein, His; AFP; Alpha-Fetoprotein
氨基酸序列

KALHENEFGIASTLDSSQCVTEKNVLSIATITFTQFVPEATEEEVNKMTSDVLAAMKKNSGDGCLESQLSVFLDEICHETELSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEPAESCKAHEENRAVFMNRFIYEVSRRNPFMYAPAILSLAAQYDKVVLACCKADNKEECFQTKRASIAKELREGSMLNEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECLQDGEKVMTYICSQQNILSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGDRNFAQFSSEEKIMFMASFLHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDNLEEELQKHIEESQALSKQSCALYQTLGDYKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVNSGISHCCNSSYSNRRLCITSFLRDETYAPPPFSEDKFIFHKDLCQAQGKALQTMKQELLINLVKQKPELTEEQLAAVTADFSGLLEKCCKAQDQEVCFTEEGPKLISKTRDALGV

分子量

65-75 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Alpha-Fetoprotein Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Alpha-Fetoprotein Protein, Mouse (HEK293, His)
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