Aminopeptidase N/APN 蛋白, Human (HEK293, His)

Aminopeptidase N/APN protein is a disulfide-bonded homotrimer functioning as a broad specificity aminopeptidase. It plays a crucial role in peptide digestion, peptide processing, antigen presentation, angiogenesis, and cholesterol crystallization. APN protein also interacts with the amino acid transporter SLC6A19 and acts as a receptor for human coronavirus 229E/HCoV-229E during infection. Aminopeptidase N/APN Protein, Human (HEK293, His) is the recombinant human-derived Aminopeptidase N/APN protein, expressed by HEK293 , with C-His labeled tag. The total length of Aminopeptidase N/APN Protein, Human (HEK293, His) is 899 a.a., with molecular weight of 130-140 kDa.

氨肽酶 N/APN 蛋白是一种广泛特异性的氨肽酶，其功能为通过二硫键连接的同源三聚体。它在胃蛋白酶和胰蛋白酶对蛋白质水解产生的肽的最终消化中起着至关重要的作用。此外，它还参与多种肽的加工，包括血管紧张素 III 和 IV 等肽激素、神经肽和趋化因子。此外，它可能参与抗原呈递细胞中与主要组织相容性复合物 II 类分子结合的肽的裂解。这种蛋白质还可能有助于血管生成并促进胆固醇结晶。此外，它作为氨基酸转运蛋白 SLC6A19 的结合伴侣，可能调节其活性并影响氨基酸转运。值得注意的是，氨基肽酶 N/APN 蛋白充当人类冠状病毒 229E/HCoV-229E 的受体，特别是在感染期间充当 HCoV-229E 刺突糖蛋白的受体。

Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2500 pmoL/min/µg, as measured under the described conditions.

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  Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is 1.3946×10⁴  pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His

P15144 (K69-K967)

290  [NCBI]

KAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK

130-140 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4-7.3.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.