Angiopoietin-2 蛋白, Human (222a.a, HEK293, His)

Angiopoietin-2 Protein, Human (222a.a, HEK293, His) is a circulating antagonistic ligand of the endothelial-specific Tie2 receptor and has been identified as an important gatekeeper of endothelial activation.

血管生成素是参与胚胎血管发育的肽。在已鉴定的四种血管生成素中，血管生成素 1 和血管生成素 2（分别为 ANGPT1 和 ANGPT2）描述得最好。由内皮细胞产生的血管生成素 2 也与 Tie2 受体结合并充当拮抗因子而不激活受体。

Measured by its binding ability in a functional ELISA. Immobilized Human Angiopoietin-2 at 1 μg/mL (100 μL/well) can bind Biotinylated TIE-2. The ED₅₀ for this effect is 11.71 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human Angiopoietin-2 at 1 μg/mL (100 μL/well) can bind Biotinylated TIE-2. The ED₅₀ for this effect is 11.71 ng/mL.

Human

HEK293

C-6*His

O15123-1 (K275-F496)

285  [NCBI]

KEEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

30-35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM MOPS, 150 mM NaCl, 10% CHAPS, pH 7.5 or 20 mM PB, 150 mM NaCl, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Angiopoietin 2.