Angiopoietin-2 蛋白, Mouse (HEK293, Fc)

Angiopoietin 2 protein (ANGPT2) binds to TEK/TIE2, competes with ANGPT1, and modulates its signaling. Even in the absence of ANGPT1, ANGPT2 induces TEK/TIE2 phosphorylation. Angiopoietin-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Angiopoietin-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Angiopoietin-2 Protein, Mouse (HEK293, Fc) is 478 a.a., with molecular weight of 90-120 kDa.

血管生成素 2 蛋白 (ANGPT2) 与 TEK/TIE2 结合并与 ANGPT1 竞争结合位点，从而调节 ANGPT1 信号传导。即使在没有 ANGPT1 的情况下，它也能够诱导 TEK/TIE2 酪氨酸磷酸化。在缺乏 VEGF 等血管生成诱导剂的情况下，ANGPT2 会导致细胞-基质接触松动，可能导致内皮细胞凋亡和随后的血管退化。然而，当与 VEGF 结合使用时，它可以促进内皮细胞迁移和增殖，使其成为允许的血管生成信号。ANGPT2还在淋巴管生成的调节中发挥作用。此外，它与 TEK/TIE2 相互作用，竞争与 ANGPT1 相同的结合位点，并与 ITGA5 相互作用。

Immobilized Mouse Angiopoietin-2 at 2 μg/mL (100 μL/well) can bind Biotinylated TIE-2. The ED₅₀ for this effect is 0.8674 μg/mL, corresponding to a specific activity is 1.15×10^3 Unit/mg.

• 
  Immobilized Mouse Angiopoietin-2 at 2 μg/mL (100 μL/well) can bind Biotinylated TIE-2. The ED₅₀ for this effect is 0.8674μg/mL, corresponding to a specific activity is 1.15×10³Unit/mg.

Mouse

HEK293

C-hFc

O35608 (Y19-F496)

11601  [NCBI]

YSNFRKSVDSTGRRQYQVQNGPCSYTFLLPETDSCRSSSSPYMSNAVQRDAPLDYDDSVQRLQVLENILENNTQWLMKLENYIQDNMKKEMVEIQQNVVQNQTAVMIEIGTSLLNQTAAQTRKLTDVEAQVLNQTTRLELQLLQHSISTNKLEKQILDQTSEINKLQNKNSFLEQKVLDMEGKHSEQLQSMKEQKDELQVLVSKQSSVIDELEKKLVTATVNNSLLQKQQHDLMETVNSLLTMMSSPNSKSSVAIRKEEQTTFRDCAEIFKSGLTTSGIYTLTFPNSTEEIKAYCDMDVGGGGWTVIQHREDGSVDFQRTWKEYKEGFGSPLGEYWLGNEFVSQLTGQHRYVLKIQLKDWEGNEAHSLYDHFYLAGEESNYRIHLTGLTGTAGKISSISQPGSDFSTKDSDNDKCICKCSQMLSGGWWFDACGPSNLNGQYYPQKQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

90-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.