Angiopoietin-2 蛋白, Rhesus macaque (HEK293, Fc)

ANGPT2 is a secreted factor on the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway and is involved in tumor angiogenesis. In the presence of ANGPT1, ANGPT2 antagonizes ANGPT1-mediated Tie2 phosphorylation, leading to vessel sprouting or degeneration depending on the level of VEGF-A. Overexpression or inhibition of ANGPT2 increases or decreases angiogenic capacity, respectively. Angiopoietin-2 Protein, Rhesus macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived Angiopoietin-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Angiopoietin-2 Protein, Rhesus macaque (HEK293, Fc) is 477 a.a., with molecular weight of 90-120 kDa.

ANGPT2 是血管生成素/Tie（具有 Ig 和 EGF 同源结构域的酪氨酸激酶）信号通路上的分泌因子，与血管生长相关的疾病有关。血管生成的驱动因素包括血管内皮细胞中的 VEGFR、血管生成素（ANG）连接、胎盘生长因子（PlGF）和转化生长因子β。Tie2 (TEK) 是 ANGPT1 和 ANGPT2 的共同受体。ANGPT1 激活 Tie2，ANGPT2 则充当 Tie2 的上下游依赖性激动剂/拮抗剂。当 ANGPT1 存在时，ANGPT2 会拮抗 ANGPT1 介导的 Tie2 磷酸化，根据 VEGF-A 的水平导致血管萌芽或退化。在缺氧环境和癌症中，ANGPT2 会从间充质干细胞和肿瘤细胞中产生。ANGPT2 主要在肿瘤中诱导血管生成，ANGPT2 的表达与微血管密度、肿瘤大小和转移疗效一致。过表达或抑制分别增加或降低血管生成能力。ANGPT2 抑制恢复了血管稳定性，抑制了肿瘤血管生成。ANGPT2 还以自分泌和旁分泌方式促进肿瘤细胞生长。ANGPT2 刺激肿瘤细胞中的 TIE2 会激活下游细胞增殖信号，ANGPT2/TIE2 轴可能为侵袭性无功能（NF）垂体神经内分泌肿瘤（PitNET），这种不可切除的肿瘤，提供了治疗靶点。据报道，MYBL1 可直接靶向 ANGPT2 启动子来增强 ANGPT2 的表达，并通过与 PRMT5、MEP50 和 WDR5 相互作用转录上调 ANGPT2 mRNA 表达。ANGPT2 还是转移性结直肠癌 (CRC) 的预后因素。

Immobilized Rhesus TIE2 His at 2 μg/ml (100 μl/well) can bind Rhesus Macaque Angiopoietin-2. The ED₅₀ for this effect is 173.6 ng/mL.

Rhesus Macaque

HEK293

C-hFc

XM_001097949 (Y19-F495)

716811  [NCBI]

YNNFRKSMDSIGKKQYQVQHGSCSYTFLLPEMDNCRSSSSPYVSNAVQRDAPLEYDDSVQRLQVLENIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLAMEDKHIIQLQSIKEEKDQLQVLVSKQNSIIEELEKKIVTATVNNSVLQKQQHDLMETVNNLLTMMSTSNSKDPTVAKEEQISFRDCAEVFKSGHTTNGVYTLTLPNSTEEVKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDADNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKGTTMMIRPADF

90-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.