1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. Angiopoietin Like 4
  5. ANGPTL4/Angiopoietin-related 4 蛋白, Human (GST)

ANGPTL4/Angiopoietin-related 4 蛋白, Human (GST)

目录号: HY-P700562
产品使用指南 技术支持

ANGPTL4 蛋白介导脂蛋白脂肪酶 (LPL) 失活,调节甘油三酯清除率和脂质代谢。它还影响葡萄糖稳态、胰岛素敏感性、内皮细胞功能和血管生成。裂解的 ANGPTL4 在 LPL 失活中更加活跃,突出了其在代谢和血管调节中的多方面作用。ANGPTL4/Angiopoietin-related 4 蛋白, Human (GST) 是重组的 ANGPTL4/血管生成素相关 4 蛋白,由 E. coli 表达,带有 N-GST 标签。

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描述

ANGPTL4 protein mediates lipoprotein lipase (LPL) inactivation, regulating triglyceride clearance and lipid metabolism. It also affects glucose homeostasis, insulin sensitivity, endothelial cell function, and angiogenesis. Cleaved ANGPTL4 is more active in LPL inactivation, highlighting its multifaceted role in metabolic and vascular regulation. ANGPTL4/Angiopoietin-related 4 Protein, Human (GST) is the recombinant human-derived ANGPTL4/Angiopoietin-related 4 protein, expressed by E. coli , with N-GST labeled tag.

研究背景

ANGPTL4/血管生成素相关 4 蛋白作为脂蛋白脂肪酶 (LPL) 失活的介质,在调节甘油三酯从血清中的清除和脂质代谢中发挥着至关重要的作用。此外,它还涉及葡萄糖稳态和胰岛素敏感性的潜在调节。ANGPTL4 抑制内皮细胞增殖、迁移和肾小管形成,从而减少血管渗漏。它还干扰内皮细胞与细胞外基质的粘附,抑制肌动蛋白细胞骨架的重组、肌动蛋白应力纤维的形成和粘着斑。在某些情况下,ANGPTL4 可能调节与肿瘤发展相关的血管生成。与未切割的蛋白相比,ANGPTL4 的切割形式在 LPL 失活中表现出更高的活性,突出了其在代谢和血管调节中的多方面作用。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

Q9BY76-1 (V28-E403)

基因 ID
蛋白结构
N-term
GST
ANGPTL4 (V28-E403)
Accession # Q9BY76-1
C-term
同用名
Angiopoietin-like protein 4Hepatic fibrinogen/angiopoietin-related protein ; HFARP
氨基酸序列

VQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSGLFEIQPQGSPPFLVNCKMTSDGGWTVIQRRHDGSVDFNRPWEAYKAGFGDPHGEFWLGLEKVHSITGDRNSRLAVQLRDWDGNAELLQFSVHLGGEDTAYSLQLTAPVAGQLGATTVPPSGLSVPFSTWDQDHDLRRDKNCAKSLSGGWWFGTCSHSNLNGQYFRSIPQQRQKLKKGIFWKTWRGRYYPLQATTMLIQPMAAE

分子量

69.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ANGPTL4/Angiopoietin-related 4 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ANGPTL4/Angiopoietin-related 4 Protein, Human (GST)
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