1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. Angiopoietin Like 4
  5. ANGPTL4/Angiopoietin-related 4 蛋白, Mouse (HEK293, His)

ANGPTL4/Angiopoietin-related 4 蛋白, Mouse (HEK293, His)

目录号: HY-P78237
产品使用指南

ANGPTL4/血管生成素相关4蛋白通过灭活脂蛋白脂肪酶(LPL)并控制血液中甘油三酯的清除,在调节脂质代谢中发挥关键作用。其作用延伸至葡萄糖稳态和胰岛素敏感性的调节。ANGPTL4/Angiopoietin-related 4 蛋白, Mouse (HEK293, His) 是重组的 ANGPTL4/血管生成素相关 4 蛋白,由 HEK293 表达,带有 N-His 标签。ANGPTL4/Angiopoietin-related 4 蛋白, Mouse (HEK293, His) 全长 243 个氨基酸,分子量为 35-50 kDa。

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描述

ANGPTL4/angiopoietin-related 4 protein plays a key role in regulating lipid metabolism by inactivating lipoprotein lipase (LPL) and controlling the clearance of triglycerides in the blood. Its effects extend to the regulation of glucose homeostasis and insulin sensitivity. ANGPTL4/Angiopoietin-related 4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ANGPTL4/Angiopoietin-related 4 protein, expressed by HEK293 , with N-His labeled tag. The total length of ANGPTL4/Angiopoietin-related 4 Protein, Mouse (HEK293, His) is 243 a.a., with molecular weight of 35-50 kDa.

研究背景

ANGPTL4/血管生成素相关 4 蛋白通过介导脂蛋白脂肪酶 (LPL) 的失活,在脂质代谢的复杂调节中发挥关键作用,从而有助于控制血液中甘油三酯的清除。除了参与脂质代谢之外,ANGPTL4 还可能在调节葡萄糖稳态和胰岛素敏感性中发挥重要作用。此外,它通过阻碍增殖、迁移和小管形成对内皮细胞发挥抑制作用,最终减少血管渗漏。体外研究表明,ANGPTL4 在异源表达时会阻碍内皮细胞与细胞外基质 (ECM) 的粘附,抑制肌动蛋白细胞骨架的重组,并破坏肌动蛋白应力纤维和粘着斑的形成。此外,与未切割的蛋白相比,ANGPTL4 的切割形式在 LPL 失活中表现出更高的活性,进一步强调了其在脂质稳态和内皮细胞功能中的多方面作用。根据具体情况,ANGPTL4 还可能调节肿瘤相关的血管生成,强调其对不同生理过程的背景影响。

种属

Mouse

表达系统

HEK293

标签

N-His

蛋白编号

Q9Z1P8 (R168-S410)

基因 ID
蛋白结构
N-term
His
ANGPTL4 (R168-S410)
Accession # Q9Z1P8
C-term
同用名
ANGPTL4; ARP4; FIAF; HFARP; Angiopoietin like 4; NGPTL2; NL2; PGAR; pp1158; ANG-3; ANG4; AGP4; ANG3; ANG-3; ANG-4; Angiopoietin-3; ANGPT4; MGC138181; MGC138183
分子量

35-50 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ANGPTL4/Angiopoietin-related 4 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ANGPTL4/Angiopoietin-related 4 Protein, Mouse (HEK293, His)
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HY-P78237
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