Animal-Free Beta-NGF 蛋白, Mouse (His)

Nerve Growth Factor-β (Beta-NGF; NGF) is a neurotrophic factor that regulates neuronal survival and differentiation. NGF is also a seminal plasma protein involved in ovulation and luteinizing. NGF has potential functions in the female reproductive system to help overcome the current problem of early embryo loss^[1][2][3]. Animal-Free Beta-NGF Protein, Mouse (His) is produced by E.coli, with C-terminal His-tag.

神经生长因子-β（Beta-NGF；NGF）是一种由 118 个氨基酸组成的碱性蛋白，作为周围神经系统的感觉和交感神经元以及前基底大脑的胆碱能神经元的营养因子^[1]。NGF 参与神经元存活和分化的调节。NGF 水平升高与创伤后应激障碍 (PTSD) 的风险相关。创伤反应导致负责 NGF 表达的 DNA 核苷酸甲基化。NGF 水平显示交感纤维密度的增加与 NGF 信使 RNA (mRNA) 水平成正比。NGF也是哺乳动物中常见的精浆蛋白。例如，NGF 作为骆驼的排卵刺激因子，并已被证明对公牛具有促黄体生成作用。NGF 在女性生殖系统中具有潜在的功能。例如，NGF在排卵诱导、LH释放、排卵、黄体发育、黄体酮（P4）产生、黄体体血管化和促性腺激素反应中发挥重要作用。对牛施用 NGF 可通过增加 LH 释放来增强类固醇产生、黄体形成和功能，并导致下游妊娠和发育标志物 mRNA 表达增加。此外，NGF 潜在的黄体生成作用有助于克服目前早期胚胎丢失的问题^[2][3]。人和牛NGF蛋白序列相似度为90.87%。同时，人NGF与大鼠和小鼠的相似性分别为85.89%和85.06%。

NGF (小鼠; 4 ng/mL; 24 h) 在成骨细胞 MC3T3-E1 中，诱导 IL-6 的产生，并增强 ALP 活性和胶原生物合成，而不影响细胞增殖^[4]。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse beta-NGF is >1 x 10⁶ IU/mg.

Mouse

E. coli

C-His

P01139 (S122-G241)

18049  [NCBI]

MSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG

Approximately 14.41 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Castellanos MR, et al. Obtención y caracterización del beta-NGF murino. Aplicación en un modelo de envejecimiento cerebral [Obtention and characterization of murine beta-NGF. Application in a model of cerebral aging]. Rev Neurol. 1998;26(153):717-722.  [Content Brief]

  [2]. Lipov EG, et al. Possible Reversal of PTSD-Related DNA Methylation by Sympathetic Blockade. J Mol Neurosci. 2017 May;62(1):67-72.  [Content Brief]

  [3]. Lima FS, et al. Insights into nerve growth factor-β role in bovine reproduction - Review. Theriogenology. 2020 Jul 1;150:288-293.  [Content Brief]

  [4]. Yada M, et al. NGF stimulates differentiation of osteoblastic MC3T3-E1 cells. Biochem Biophys Res Commun. 1994 Dec 15;205(2):1187-93.  [Content Brief]