Animal-Free BMP-10 蛋白, Human (His)

BMP-10 is essential for embryonic cardiomyocyte proliferation, preventing premature activation of CDKN1C/p57KIP and ensuring optimal expression of MEF2C and NKX2-5.As a ligand for AVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, it activates SMAD1, SMAD5 and SMAD8, regulating key signaling pathways.Animal-Free BMP-10 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-10 protein, expressed by E.coli , with C-His labeled tag.

BMP-10 对于维持胚胎心肌细胞的增殖活性、防止负性细胞周期调节因子 CDKN1C/p57KIP 的过早激活以及确保 MEF2C 和 NKX2-5 等心肌因子的必要表达水平至关重要。作为 AVRL1/ALK1、BMPR1A/ALK3 和 BMPR1B/ALK6 的配体，它触发 SMAD1、SMAD5 和 SMAD8 转录因子的激活，协调关键的信号传导通路。此外，BMP-10 对内皮细胞迁移和生长具有抑制作用，在乳腺癌细胞系中，它可能会减弱细胞迁移和细胞基质粘附。在结构上，它形成通过二硫键连接的同二聚体。值得注意的是，BMP-10 通过其 N 末端结构域与细胞外基质蛋白 FBN1 和 FBN2 相互作用，并与 ENG 结合，进一步强调了其在各种细胞过程中的多方面作用。

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 1.7-2.1 ng/mL.

Human

E. coli

C-His

O95393 (N317-R424)

27302  [NCBI]

MNAKGNYCKRTPLYIDFKEIGWDSWIIAPPGYEAYECRGVCNYPLAEHLTPTKHAIIQALVHLKNSQKASKACCVPTKLEPISILYLDKGVVTYKFKYEGMAVSECGCR

Approximately 13.10 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.