Animal-Free BMP-2 蛋白, Human/Mouse/Rat (His)

BMP-2 protein is an important member of the TGF-β superfamily and is critical in cardiogenesis, neurogenesis, and osteogenesis, inducing cartilage and bone formation. It initiates canonical BMP signaling by binding to BMPR1A and BMPR2, triggering BMPR2 phosphorylation and SMAD1/5/8 activation for gene transcription regulation. Animal-Free BMP-2 Protein, Human/Mouse/Rat (His) is the recombinant human-derived animal-Free BMP-2 protein, expressed by E. coli , with C-His labeled tag.

BMP-2 蛋白是 TGF-β 超家族的重要成员，在多种发育过程中发挥着重要作用，包括心脏生成、神经生成和骨生成。它诱导软骨和骨形成，并通过与 I 型受体 BMPR1A 和 II 型受体 BMPR2 结合启动典型的 BMP 信号级联。这种复合物的形成触发 BMPR2 磷酸化，激活 BMPR1A，进而磷酸化 SMAD1/5/8 以调节基因转录。BMP-2 还参与非经典途径，例如 ERK/MAP 激酶信号级联，影响成骨细胞分化。此外，它还通过 EIF2AK3-EIF2A-ATF4 途径刺激成肌细胞分化为成骨细胞。作为成牙本质细胞分化的正调节因子，BMP-2 形成同二聚体并与多种蛋白质相互作用，包括 SOSTDC1、GREM2、RGMA、RGMB、RGMC、ASPN、FBN1、FBN2、SCUBE3、TNFAIP6 和 ERFE。其复杂的相互作用凸显了其在多个细胞过程中的多功能调节作用。

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <9.5 ng/mL. The specific activity of recombinant BMP-2 is > 3.2 x 10⁶ IU/mg

Human

E. coli

C-His

P12643 (Q283-R396)

650  [NCBI]

MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

Approximately 13.76 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.