Animal-Free BMP-6 蛋白, Human (His)

BMP-6 protein is a member of the TGF-β superfamily and is critical in various developmental processes including skeletal development. It acts as a key regulator of HAMP/hepcidin expression and iron metabolism via hemojuvelin/HJV. Animal-Free BMP-6 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-6 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free BMP-6 Protein, Human (His) is 117 a.a., with molecular weight of ~14.07 kDa.

BMP-6 蛋白是 TGF-β 超家族的成员，在各种发育过程中不可或缺，包括软骨和骨形成。除了在骨骼发育中的作用外，BMP-6 还通过充当血幼素/HJV 的配体，充当 HAMP/铁调素表达和铁代谢的重要调节剂。此外，它可能通过与其受体 BMPR1A/ALK3 相互作用来促进 HAMP 表达。典型 BMP 信号级联的启动涉及 BMP-6 与 I 型受体 ACVR1 和 II 型受体 ACVR2B 结合，ACVR1 通过磷酸化 SMAD1/5/8 传播信号，这些信号进入细胞核并充当 BMP 转录的激活剂和阻遏剂。目标基因。此外，BMP-6 可以参与非经典途径，例如 TAZ-Hippo 信号级联，通过调节 VEGFR2 表达来影响 VEGF 信号传导。它与多种蛋白质形成相互作用，包括 SOSTDC1、Hemojuvelin/HJV、ERFE 和 BMPR1A/ALK3，展示了其在不同细胞过程中的多功能调节作用。

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <87 ng/mL

Human

E. coli

C-His

P22004 (V397-H513)

654  [NCBI]

MVSSASDYNSSELKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCH

Approximately 14.07 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5 or PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.