Animal-Free FGF-19 蛋白, Human (His)

FGF-19 protein plays a crucial role in regulating bile acid biosynthesis by inhibiting CYP7A1 expression through positive regulation of JNK and ERK1/2 cascades. It stimulates glucose uptake in adipocytes, dependent on KLB and FGFR4. Animal-Free FGF-19 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-19 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free FGF-19 Protein, Human (His) is 194 a.a., with molecular weight of ~22.62 kDa.

FGF-19 蛋白通过正调节 JNK 和 ERK1/2 级联抑制 CYP7A1 表达，在胆汁酸生物合成的调节中发挥关键作用。此外，FGF-19 刺激脂肪细胞中的葡萄糖摄取，其活性取决于 KLB 和 FGFR4 的存在。该蛋白与 FGFR1、FGFR2、FGFR3 和 FGFR4 形成重要的相互作用，强调其参与 FGF 受体信号传导途径。FGF 与其受体之间的亲和力通过 KL、KLB 和硫酸乙酰肝素糖胺聚糖（作为辅助受体）增强。FGF-19 直接与 KL 和 KLB 相互作用，并且在肝素、KL 或 KLB 存在的情况下，它与 FGFR4 形成相互作用。此外，FGF-19 与 MALRD1 相互作用，突出了其多样化的分子关联，并表明其参与了复杂的细胞过程。

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <51 ng/mL.

Human

E. coli

C-His

O95750 (R23-K216)

9965  [NCBI]

MRPLAFSDAGPHVHYGWGDPIRLRHLYTSGPHGLSSCFLRIRADGVVDCARGQSAHSLLEIKAVALRTVAIKGVHSVRYLCMGADGKMQGLLQYSEEDCAFEEEIRPDGYNVYRSEKHRLPVSLSSAKQRQLYKNRGFLPLSHFLPMLPMVPEEPEDLRGHLESDMFSSPLETDSMDPFGLVTGLEAVRSPSFEK

Approximately 22.62 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.