Animal-Free FGF-4 蛋白, Human (His)

The FGF-4 protein coordinates embryonic development, cell proliferation and differentiation and is critical for normal limb and heart valve development. FGF-4 may promote embryonic molar tooth bud development by inducing key gene expression. Animal-Free FGF-4 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-4 protein, expressed by E. coli , with C-His labeled tag.

FGF-4 蛋白在协调胚胎发育、细胞增殖和细胞分化中发挥着关键作用。它在胚胎发生过程中四肢和心脏瓣膜的正常发育中是不可或缺的。此外，FGF-4可能通过诱导牙齿间充质细胞中关键基因（包括MSX1、MSX2和SDC1）的表达来促进胚胎磨牙牙芽的发育，从而凸显其多样化的调节功能。FGF-4 与 FGFR1、FGFR2、FGFR3 和 FGFR4 进行复杂的相互作用，形成对信号级联至关重要的分子联盟。硫酸乙酰肝素糖胺聚糖增强了 FGF-4 与其受体之间的结合亲和力，在这个复杂的调控网络中充当了不可或缺的辅助受体。这些相互作用强调了 FGF-4 在驱动发育过程中的基本过程中的多方面和重要作用。

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2.5 ng/mL. The specific activity of recombinant human FGF-4 is >4 x 10⁵ IU/mg.

Human

E. coli

C-His

P08620-1 (G25-L206)

2249  [NCBI]

MGRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL

Approximately 20.70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 0.1% sarkosyl in 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.