Animal-Free GDF-5 蛋白, Human (His)

The GDF-5 protein is critical in bone and cartilage formation, complexly regulating chondrogenic tissue differentiation through dual pathways. It positively affects cartilage formation by inducing SMAD protein signaling by binding to BMPR1B and BMPR1A. Animal-Free GDF-5 Protein, Human (His) is the recombinant human-derived animal-FreeGDF-5 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free GDF-5 Protein, Human (His) is 120 a.a., with molecular weight of ~14.52 kDa.

GDF-5 蛋白在骨和软骨形成中发挥着关键作用，通过双重途径复杂地调节软骨形成组织分化。首先，它通过与 BMPR1B 高亲和力结合和与 BMPR1A 低亲和力结合，对软骨形成组织分化产生积极影响，从而诱导 SMAD1-SMAD5-SMAD8 复合物磷酸化和随后的 SMAD 蛋白信号转导。同时，它通过与 NOG 相互作用来负向调节软骨形成分化。这种蛋白质对于防止去神经支配时肌肉过度流失至关重要，这是一种由磷酸化 SMAD1/5/8 介导的功能。此外，GDF-5 可结合细菌脂多糖 (LPS) 并介导 LPS 诱导的炎症反应，包括单核细胞分泌 TNF。在结构上，它形成通过二硫键连接的同二聚体，并与丝氨酸蛋白酶 HTRA1 和 HTRA3 相互作用。LPS 结合后，GDF-5 可能与 CXCR4、HSP90AA1 和 HSPA8 形成复合物。此外，它与 NOG 具有高亲和力相互作用，抑制软骨形成，并与 BMPR1B 和 BMPR1A 相互作用，通过 SMAD 依赖性信号传导正向调节软骨细胞分化。此外，它还与 FBN1 和 FBN2 相互作用。

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <14 ng/mL

Human

E. coli

C-His

P43026 (A382-R501)

8200  [NCBI]

MAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR

Approximately 14.52 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.