1. 重组蛋白
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Animal-Free HDGF 蛋白, Mouse (His)

Animal-Free HDGF 蛋白, Mouse (His)

目录号: HY-P700181AF
COA 产品使用指南

HDGF 蛋白是一种转录抑制因子,可促进成纤维细胞有丝分裂活性。它以单体或结构域交换同二聚体形式存在,与核蛋白 NCL 和 YBX1/YB1 相互作用。Animal-Free HDGF 蛋白, Mouse (His) 是重组的 非动物源 HDGF 蛋白,由 E. coli 表达,带有 C-His 标签。Animal-Free HDGF 蛋白, Mouse (His) 全长 237 个氨基酸,分子量约为 ~27.08 kDa。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1500 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

* Please select Quantity before adding items.

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描述

HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. Animal-Free HDGF Protein, Mouse (His) is the recombinant mouse-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free HDGF Protein, Mouse (His) is 237 a.a., with molecular weight of ~27.08 kDa.

研究背景

HDGF 蛋白具有转录抑制因子的功能,并对成纤维细胞具有促有丝分裂活性。这种肝素结合蛋白可以单体和结构域交换的同二聚体的形式存在。此外,它还与核蛋白 NCL 和 YBX1/YB1 相互作用。

种属

Mouse

表达系统

E. coli

标签

C-His

蛋白编号

P51859 (M1-L237)

基因 ID
蛋白结构
N-term
HDGF (M1-L237)
Accession # P51859
His
C-term
同用名
Hepatoma-Derived Growth Factor; HDGF; High Mobility Group Protein 1-Kike 2; HMG-1L2; HDGF; HMG1L2
氨基酸序列

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL

分子量

Approximately 27.08 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Animal-Free HDGF Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free HDGF Protein, Mouse (His)
目录号:
HY-P700181AF
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