Animal-Free IFN-lambda 3/IL-28B 蛋白, Mouse (His)

The IFN-lambda 3/IL-28B protein is a cytokine with antiviral, antitumor, and immunomodulatory properties that is critical for defense against viruses, especially in epithelial tissues. It acts as a ligand for IL10RB and IFNLR1 receptor complexes, activating the JAK/STAT signaling pathway and inducing IFN-stimulated genes (ISG) into an antiviral state. Animal-Free IFN-lambda 3/IL-28B Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIFN-lambda 3/IL-28B protein, expressed by E. coli , with C-His labeled tag.

IFN-lambda 3/IL-28B 蛋白是一种细胞因子，具有抗病毒、抗肿瘤和免疫调节特性。它在宿主抵御病毒的过程中发挥着至关重要的作用，特别是在上皮组织中。该蛋白作为 IL10RB 和 IFNLR1 受体复合物的配体，导致 JAK/STAT 信号通路的激活以及有助于抗病毒状态的 IFN 刺激基因 (ISG) 的表达。其受体主要在上皮细胞中表达，这解释了其细胞类型选择性作用。尽管它对于阴道感染的早期宿主防御可能不是必需的，但它在 Toll 样受体 (TLR) 诱导的抗病毒防御和肠上皮的免疫防御中发挥着重要作用。此外，IFN-lambda 3/IL-28B 蛋白还通过上调 MHC I 类抗原表达发挥免疫调节作用。

Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <20 ng/mL.

Mouse

E. coli

C-His

Q8CGK6 (D20-V193)

338374  [NCBI]

MDPVPRATRLPVEAKDCHIAQFKSLSPKELQAFKKAKGAIEKRLLEKDMRCSSHLISRAWDLKQLQVQERPKALQAEVALTLKVWENINDSALTTILGQPLHTLSHIHSQLQTCTQLQATAEPKPPSRRLSRWLHRLQEAQSKETPGCLEDSVTSNLFQLLLRDLKCVASGDQCV

Approximately 20.56 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.