Animal-Free IL-1RA/IL-1RN 蛋白, Human (His)

IL-1RA/IL-1RN proteins are potent anti-inflammatory antagonists in the interleukin 1 family, specifically targeting the proinflammatory cytokines IL1B and IL1A. It protects against immune dysregulation and, crucially, prevents IL1 from triggering uncontrolled systemic inflammation in response to various innate stimulants, including pathogens. Animal-Free IL-1RA/IL-1RN Protein, Human (His) is the recombinant human-derived animal-FreeIL-1RA/IL-1RN protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-1RA/IL-1RN Protein, Human (His) is 152 a.a., with molecular weight of ~18.07 kDa.

IL-1RA/IL-1RN 蛋白是 IL-1 家族中的一种有效抗炎拮抗剂，专门针对促炎细胞因子，如 IL-1β/IL1B 和 IL-1α/IL1A。作为防止免疫失调的保障措施，这种蛋白质有助于防止 IL1 响应各种先天刺激剂（包括病原体）而引发不受控制的全身炎症。其不含动物成分的性质强调了其在研究和治疗应用中的实用性，为研究和减轻炎症过程提供了可靠且合乎道德的工具，而无需依赖动物源性材料。

Measure by its ability to inhibit IL-1 alpha-dependent proliferation in D10.G4.1 cells. The ED₅₀ for this effect is <50 ng/mL.

Human

E. coli

C-His

P18510-1 (R26-E177)

3557  [NCBI]

MRPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQEDE

Approximately 18.07 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.