Animal-Free IL-2 蛋白, Pig (His)

IL-2 is produced by antigen-activated CD4+ T cells, CD8+ T cells, NK cells and NKT cells. IL-2 is involved in signaling pathways including JAK/STAT, inosine phosphate 3-kinase /PI3K and mitogen-activated protein kinase /MAPK. IL-2 can be used in the research of cancer immunotherapy. Animal-Free IL-2 Protein, Pig (His) is the recombinant pig-derived animal-FreeIL-2 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-2 Protein, Pig (His) is 134 a.a., with molecular weight of ~16.2 kDa.

IL-2 是一种受体细胞因子，由活化的 CD4 阳性辅助性 T 细胞产生，通过激活 JAK/STAT、磷酸肌苷-3-激酶/PI3K 和丝裂原活化蛋白激酶/MAPK 等多种途径发挥作用。IL-2 可结合由高亲和三聚体IL-2R (IL2RA/CD25, IL2RB/CD122和IL2RG/CD132) 或低亲和二聚体 IL-2R (IL2RB和IL2RG) 组成的受体复合物。IL-2 可增加 NK 细胞的细胞溶解活性。促进活化 B 细胞的强增殖和免疫球蛋白的产生。IL-2 参与效应 T 细胞亚群的分化和稳态，包括 Th1、Th2、Th17 以及记忆 CD8 阳性 T 细胞。IL-2 的合成在 mRNA 水平上受到 TCR 和 CD28 信号的严格调控。可介导激活诱导的细胞死亡 (AICD) 过程。IL-2 可用于癌症免疫治疗的研究。

Measure by its ability to induce proliferation in CTLL2 cells. The ED₅₀ for this effect is < 0.5 ng/mL.

Pig

E. coli

C-His

P26891(A21-T154)

396868  [NCBI]

MAPTSSSTKNTKKQLEPLLLDLQLLLKEVKNYENADLSRMLTFKFYMPKQATELKHLQCLVEELKALEGVLNLGQSKNSDSANIKESMNNINVTVLELKGSETSFKCEYDDETVTAVEFLNKWITFCQSIYSTLT

Approximately 16.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.