2. 重组蛋白
  3. Cytokines and Growth Factors Animal-free Recombinant Proteins
  4. Animal-Free Laminin 521 蛋白, Human

Animal-Free Laminin 521 蛋白, Human

目录号: HY-P701311AF
COA 产品使用指南

Laminins are a major component of basement membranes. Laminin 521 is a heterotrimeric glycoprotein composed of one α5, one β2, and one γ1 chain, is a defined matrix for feeder-free culture of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human ESCs and iPSCs without spontaneous differentiation.

无动物成分 (Animal-free) 重组蛋白，即生产过程中不使用或接触任何动物来源的材料和试剂，具有高一致性和稳定性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
100 μg ￥1700 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

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描述

Laminins are a major component of basement membranes. Laminin 521 is a heterotrimeric glycoprotein composed of one α5, one β2, and one γ1 chain, is a defined matrix for feeder-free culture of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human ESCs and iPSCs without spontaneous differentiation.
种属

Human
表达系统

HEK293
蛋白编号

/
基因 ID

/
同用名
Laminin 521
分子量

90-110 kDa, 40-50 kDa, 27- 30 kDa
纯度

Greater than 95% as determined by SEC-HPLC.

性状

溶液
组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 or PBS,10% Glycerol,pH7.4.
内毒素含量

<0.01 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (208 KB)

产品使用指南 (1213 KB)

Animal-Free Laminin 521 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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