Animal-Free LIF 蛋白, Human (His)

LIF protein induces terminal differentiation in leukemic cells, promoting hematopoietic and neuronal cell differentiation, and stimulating acute-phase protein synthesis in hepatocytes. Its diverse activities highlight its role in influencing cellular differentiation across various cell types and physiological contexts. Animal-Free LIF Protein, Human (His) is the recombinant human-derived animal-FreeLIF protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free LIF Protein, Human (His) is 180 a.a., with molecular weight of ~20.52 kDa.

LIF 蛋白具有诱导白血病细胞终末分化的能力。其多种活性包括诱导正常细胞和髓性白血病细胞的造血分化、促进神经元细胞分化以及刺激肝细胞中的急性期蛋白质合成。这些多方面的活动强调了 LIF 在影响各种细胞类型和生理环境的细胞分化中的作用。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL.

Human

E. coli

N-His

P15018 (S23-F202)

3976  [NCBI]

SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF

Approximately 20.52 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.