1. 重组蛋白
  2. Enzymes & Regulators Animal-free Recombinant Proteins
  3. Matrix Metalloproteinases
  4. MMP-2
  5. Animal-Free MMP-2 蛋白, Human (His)

Animal-Free MMP-2 蛋白, Human (His)

目录号: HY-P700139AF
COA 产品使用指南

MMP-2蛋白是一种多功能金属蛋白酶,积极参与血管重塑、血管生成、组织修复、肿瘤侵袭、炎症和动脉粥样硬化斑块破裂等生理过程。除了降解细胞外基质蛋白外,它还作用于非基质蛋白,促进血管收缩。Animal-Free MMP-2 蛋白, Human (His) 是重组的 无动物源 MMP-2 蛋白,由 E. coli 表达,带有 C-His 标签。Animal-Free MMP-2 蛋白, Human (His) 全长 551 个氨基酸,分子量约为 ~63.00 kDa。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
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2 μg ¥720 In-stock
10 μg ¥2000 In-stock
50 μg ¥5600 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MMP-2 protein is a multifunctional metalloproteinase that actively participates in physiological processes such as vascular remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. In addition to degrading extracellular matrix proteins, it also acts on non-matrix proteins to promote vasoconstriction. Animal-Free MMP-2 Protein, Human (His) is the recombinant human-derived animal-FreeMMP-2 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free MMP-2 Protein, Human (His) is 551 a.a., with molecular weight of ~63.00 kDa.

研究背景

MMP-2 蛋白是一种普遍存在的金属蛋白酶,积极参与一系列生理过程,包括脉管系统重塑、血管生成、组织修复、肿瘤侵袭、炎症和动脉粥样硬化斑块破裂。除了降解细胞外基质蛋白的作用外,该蛋白还通过作用于非基质蛋白(例如大内皮 1 和 β 型 CGRP)而表现出多功能性,从而促进血管收缩。此外,它在 Gly-|-Leu 键处裂解 KISS,并且似乎在心肌细胞死亡途径中发挥作用。通过调节 GSK3beta 的活性并在体外裂解 GSK3beta,它有助于心肌氧化应激。MMP-2 与 MMP14 相关,参与纤维血管组织的形成。值得注意的是,MMP-2 的 C 端非催化片段(称为 PEX)具有抗血管生成和抗肿瘤特性,可抑制细胞迁移和与 FGF2 和玻连蛋白的粘附。此外,它还充当血管表面整合素 α-v/β3 的配体。

生物活性

Measured by its ability to cleave 20µM fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 that incubate at room temperature in kinetic mode for 5 minutes .The specific activity is 855.17pmol/min/µg.

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

P08253 (Y110-C660)

基因 ID
蛋白结构
N-term
MMP-2 (Y110-C660)
Accession # P08253
His
C-term
同用名
rHu72 kDa type IV collagenase/MMP-2, His ; 72 kDa Type IV Collagenase; 72 kDa Gelatinase; Gelatinase A; Matrix Metalloproteinase-2; MMP-2; TBE-1; MMP2; CLG4A
氨基酸序列

MYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC

分子量

Approximately 63.00 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0, trehalose.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Animal-Free MMP-2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free MMP-2 Protein, Human (His)
目录号:
HY-P700139AF
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