Animal-Free PF-4/CXCL4 蛋白, Human (His)

The PF-4/CXCL4 protein released during platelet aggregation has a crucial impact on physiological processes. It neutralizes the anticoagulant effect of heparin with higher affinity than chondroitin 4-sulfate chains. Animal-Free PF-4/CXCL4 Protein, Human (His) is the recombinant human-derived animal-FreePF-4/CXCL4 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free PF-4/CXCL4 Protein, Human (His) is 70 a.a., with molecular weight of ~8.58 kDa.

血小板聚集过程中释放的PF-4/CXCL4蛋白在各种生理过程中发挥着关键作用。与载体分子的 4-硫酸软骨素链相比，它对肝素表现出更高的结合亲和力，从而中和肝素的抗凝作用。此外，PF-4 具有趋化特性，可吸引中性粒细胞和单核细胞，从而促进免疫反应。值得注意的是，它还起到内皮细胞增殖抑制剂的作用，较短的形式比较长的变体显示出更大的效力。研究证明，从结构上看，PF-4 形成同四聚体。此外，它通过其 Link 结构域与 TNFAIP6 结合，强调其参与复杂的分子相互作用。这种多方面的功能强调了 PF-4/CXCL4 在调节止血、免疫反应和血管过程中的重要性。

Measure by its ability to inhibit human FGF-2-induce proliferation in HUVEC cells. The ED₅₀ for this effect is <5 μg/mL.

Human

E. coli

N-His

P02776 (E32-S101)

5196  [NCBI]

EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

Approximately 8.58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.