Animal-Free TGF beta 3/TGFB3 蛋白, Human (His)

Latent transforming growth factor beta-3 (TGF-beta-3) preprotein serves as a precursor to latency-associated peptide (LAP) and active TGF-beta-3 chains, which serve as regulatory and functional subunits. It plays a crucial role in maintaining the latent state of TGF-β-3 within the extracellular matrix. Animal-Free TGF beta 3/TGFB3 Protein, Human (His) is the recombinant human-derived animal-FreeTGF beta 3/TGFB3 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free TGF beta 3/TGFB3 Protein, Human (His) is 112 a.a., with molecular weight of ~13.66 kDa.

潜在转化生长因子 β-3 (TGF-β-3) 前蛋白是潜伏相关肽 (LAP) 和活性 TGF-β-3 链的前体，分别充当调节亚基和功能亚基。它在维持细胞外基质内 TGF-β-3 的潜伏状态方面发挥着至关重要的作用。通过与 TGF-β-3 的非共价结合，潜在 TGF-β-3 通过与 LTBP1 和 LRRC32/GARP 等关键“环境分子”相互作用，主动调节激活过程。这些相互作用有助于 TGF-β-3 的受控激活，其中 LTBP1 和 LRRC32/GARP 是该调节机制中的关键组成部分。此外，与整合素的相互作用会诱导潜伏期相关肽链的结构变化，导致随后释放活性 TGF-β-3。这种复杂的分子相互作用强调了潜在 TGF-β-3 在各种生理环境中协调 TGF-β-3 的调节激活中的关键作用。

Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2x10⁷ lU/mg.

Human

E. coli

C-His

P10600 (A301-S412)

7043  [NCBI]

MALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS

Approximately 13.66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 0.2 M NaCl, 20 mM sodium citrate, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.