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  3. ANKFY1 蛋白, Human (Sf9, His, Strep)

ANKFY1 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701530
产品使用指南

ANKFY1 是一种拟议的 Rab5 效应子,通过与 PI(3)P 结合,促进同型早期内体融合并参与异型融合,从而关键参与内体动力学。它在巨胞饮作用中发挥着至关重要的作用,依赖于 Rab5-GTP,并且对于激活的酪氨酸激酶受体(例如 PDGFRB)的正确定位至关重要。ANKFY1 蛋白, Human (Sf9, His, Strep) 是重组的 ANKFY1 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。ANKFY1 蛋白, Human (Sf9, His, Strep) 全长 1168 个氨基酸。

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描述

ANKFY1 is a proposed Rab5 effector that critically participates in endosomal dynamics by binding to PI(3)P, promoting homotypic early endosomal fusion and participating in heterotypic fusion. It plays a crucial role in macropinocytosis, is dependent on Rab5-GTP, and is essential for the correct localization of activated tyrosine kinase receptors such as PDGFRB. ANKFY1 Protein, Human (Sf9, His, Strep) is the recombinant human-derived ANKFY1 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of ANKFY1 Protein, Human (Sf9, His, Strep) is 1168 a.a., .

研究背景

ANKFY1 是 Rab5 的一种拟议效应子,通过与磷脂酰肌醇 3-磷酸 (PI(3)P) 结合并促进同型早期内体融合,在内体动力学中发挥着至关重要的作用。它还参与网格蛋白包被的囊泡与早期内体的异型融合,尽管程度较小。ANKFY1 与巨胞饮作用密切相关,这是一个依赖于 Rab5-GTP 的过程。此外,ANKFY1 对于激活的酪氨酸激酶受体(例如 PDGFRB)的正确内体定位和内化至关重要。值得注意的是,它以 EHD1 依赖性方式调节逆转录酶复合物的亚细胞定位,影响内体到高尔基体的转运以及到晚期内体和溶酶体的生物合成转运。与 RAB5A、RHOD、EHD1 和 VPS26A 等关键蛋白的相互作用强调了 ANKFY1 在协调内体和逆行转运过程各个方面的作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

Q9P2R3 (A2-S1169)

基因 ID

51479

蛋白结构
N-term
8*His-Strep
ANKFY1 (A2-S1169)
Accession # Q9P2R3
C-term
同用名
ANKFY1; Rabankyrin-5; Rank-5; Ankyrin repeat and FYVE domain-containing protein 1; Ankyrin repeats hooked to a zinc finger motif
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ANKFY1 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ANKFY1 Protein, Human (Sf9, His, Strep)
目录号:
HY-P701530
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