Antithrombin III/Serpin C1 蛋白, Human (432a.a, HEK293, His)

Antithrombin III/Serpin C1 protein, the main plasma serine protease inhibitor, regulates the blood coagulation cascade. It inhibits thrombin, matriptase-3/TMPRSS7, and factors IXa, Xa, and XIa, with enhanced potency in the presence of heparin. Furthermore, it forms a protease-inhibiting heterodimer with TMPRSS7. Antithrombin III/Serpin C1 Protein, Human (432a.a, HEK293, His) is the recombinant human-derived Antithrombin III/Serpin C1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Antithrombin III/Serpin C1 Protein, Human (432a.a, HEK293, His) is 432 a.a., with molecular weight of 55-60 kDa.

抗凝血酶 III/丝氨酸蛋白酶抑制剂 C1 蛋白是血浆中主要的丝氨酸蛋白酶抑制剂，在调节凝血级联中起着至关重要的作用。它能有效抑制凝血酶、matriptase-3/TMPRSS7 和因子 IXa、Xa 和 XIa，其抑制活性在肝素存在下显着增强。此外，抗凝血酶 III/丝氨酸蛋白酶抑制剂 C1 与 TMPRSS7 形成蛋白酶抑制异二聚体。

Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC and the IC₅₀ value is < 5 nM.

Human

HEK293

C-His

P01008 (S35-K464)

462  [NCBI]

SPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK

55-60 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.