Apolipoprotein H/APOH 蛋白, Human (HEK293, His)

Apolipoprotein H Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein H (ApoH) is the main target of anti-phospholipid antibodies (aPLs) found in many patients with systemic lupus erythematosus and antiphospholipid syndrome (APS)^[1].

载脂蛋白 H (ApoH)，也称为 β2-糖蛋白 I (β2GPI)，是一种 43-50 kDa 单链糖蛋白，主要在肝脏中表达，但也在其他细胞类型中表达，例如内皮细胞、淋巴细胞、星形胶质细胞和神经元。ApoH 是一种磷脂结合（例如心磷脂）血浆蛋白，还可以结合其他带负电的分子（例如 DNA 和氧化低密度脂蛋白），并与多种蛋白质相互作用^[1]。

Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.4957 μg/mL, corresponding to a specific activity is 2.02×10³ units/mg.

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  Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.4957 μg/mL, corresponding to a specific activity is 2.02×10³ units/mg.

Human

HEK293

C-His

P02749 (G20-C345)

350  [NCBI]

GRTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPCHHHHHH

45-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Mather KA, et al. Genome-wide significant results identified for plasma apolipoprotein H levels in middle-agedand older adults. Sci Rep. 2016 Mar 31;6:23675.  [Content Brief]