2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. APT 蛋白, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc)

APT 蛋白, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc)

目录号: HY-P72047
产品使用指南

APT，也称为腺嘌呤磷酸核糖转移酶，在形成 AMP（单磷酸腺苷）的救援反应中发挥着至关重要的作用。这种补救途径为 AMP 合成提供了比从头合成更节能的途径。APT 蛋白, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc) 是重组的 APT 蛋白，由 Sf9 insect cells 表达，带有 N-10*His, C-Myc 标签。APT 蛋白, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc) 全长 172 个氨基酸，分子量约为 ~22.7 kDa。

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描述

APT, also known as adenine phosphoribosyltransferase, plays a crucial role in the rescue reaction that forms AMP (adenosine monophosphate). This salvage pathway provides a more energy-efficient route for AMP synthesis than de novo synthesis. APT Protein, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc) is the recombinant APT protein, expressed by Sf9 insect cells , with N-10*His, C-Myc labeled tag. The total length of APT Protein, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc) is 172 a.a., with molecular weight of ~22.7 kDa.
研究背景

APT，也称为腺嘌呤磷酸核糖基转移酶，在形成 AMP（单磷酸腺苷）的挽救反应中发挥着至关重要的作用。与从头合成相比，这种补救途径为 AMP 合成提供了一种能量效率更高的途径。通过催化磷酸核糖基从 PRPP（5-磷酸核糖基-1-焦磷酸）转移到腺嘌呤，APT 有助于腺嘌呤核苷酸的回收和利用，确保其在各种细胞过程中的可用性。了解 APT 介导的挽救反应的精确机制和调节可以深入了解核苷酸代谢和细胞能量平衡的维持。
种属

Others
表达系统

Sf9 insect cells
标签

N-10*His;C-Myc
蛋白编号

P63546 (M1-G172)
基因 ID

/
蛋白结构
N-term
10*His
APT (M1-G172)
Accession # P63546
Myc
C-term
同用名
apt; SPy_0927; M5005_Spy0728Adenine phosphoribosyltransferase; APRT; EC 2.4.2.7
氨基酸序列

MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYACDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLTMHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIRNYDYKVLMQFPG

分子量

Approximately 22.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

APT Protein, Streptococcus pyogenes serotype M1 (Baculovirus, His-Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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