ARMET/MANF 蛋白, Mouse (HEK293, His)

ARMET/MANF proteins regulate the survival of dopaminergic neurons in the ventral midbrain and modulate GABAergic transmission to dopaminergic neurons in the substantia nigra. It enhances GABA inhibitory currents and inhibits cell proliferation while preventing ER stress-induced cell death. ARMET/MANF Protein, Mouse (HEK293, His) is the recombinant mouse-derived ARMET/MANF protein, expressed by HEK293 , with C-His labeled tag.

ARMET/MANF 蛋白是腹侧中脑多巴胺能神经元存活的重要调节因子，同时还调节 GABA 能向黑质多巴胺能神经元的传输。它增强这些神经元中自发的和诱发的 GABA 能抑制性突触后电流。此外，它还能抑制细胞增殖并防止内质网应激引起的细胞死亡。在正常情况下，ARMET/MANF 蛋白通过与伴侣蛋白 HSPA5 结合而保留在 ER/肌浆网中。然而，它是由 ER/SR 响应 ER 应激和缺氧而上调和分泌的。分泌后，它直接与 3-O-磺基半乳糖神经酰胺（靶细胞外细胞膜上的脂硫苷）结合，促进其通过内吞作用被摄取。这种脑硫脂结合对于 ARMET/MANF 在缺氧和 ER 应激条件下减轻 ER 应激和细胞毒性的作用至关重要。ARMET/MANF 蛋白还与 HSPA5 相互作用，并且是包含 CRELD2、MATN3 和 PDIA4 的复合物的组成部分。

Measured in a cell proliferation assay using rat C6 cells. The ED₅₀ for this effect is 1.457 μg/mL.

Mouse

HEK293

C-6*His

Q9CXI5/NP_083379.2 (L22-L179)

74840  [NCBI]

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGEMCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Approximately 20 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.