1. 重组蛋白
  2. CD Antigens
  3. Erythrocyte CD Proteins Endothelial cell CD Proteins
  4. CD297/ART4
  5. ART4/CD297 蛋白, Cynomolgus (HEK293, His)

ART4/CD297 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P77298
COA 产品使用指南

ART4/CD297 蛋白属于 Arg 特异性 ADP-核糖基转移酶家族。ART4/CD297 蛋白, Cynomolgus (HEK293, His) 是重组的 ART4/CD297 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

ART4/CD297 Protein belongs to the Arg-specific ADP-ribosyltransferase family. ART4/CD297 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ART4/CD297 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

ART4/CD297 蛋白是一种含有单 ADP 核糖基化 (ART) 基序的蛋白质。胱抑素 B/CSTB 蛋白是 ADP-核糖基转移酶基因家族的成员,但酶活性尚未经过实验证明。Dombrock 血型系统的抗原位于半胱氨酸蛋白酶抑制剂 B/CSTB 蛋白上,该蛋白是糖基磷脂酰肌醇,锚定在红细胞膜上。

种属

Cynomolgus

表达系统

HEK293

标签

C-6*His

蛋白编号

G7PJY0 (T31-K300)

基因 ID

/

蛋白结构
N-term
ART4 (T31-K300)
Accession # G7PJY0
His
C-term
同用名
Ecto-ADP-ribosyltransferase 4; ARTC4; Mono(ADP-ribosyl)transferase 4; DO; DOK1
氨基酸序列

TAAPTVIRIWLLGGLLPFLLLLSGLQRPTDGSEVAMKIDFDFAPGSFDDQYQGCSKEVMEKLTQGDYFTKDIEAQKNYFGMWQKAHLTWLNQGKVLPQNMTTTHAVAILFYTLNSNVRSNFTRAVAYVARAPQQYERSFQFKYLHYYLTTAVQLLRKDSVMKNGTPLCYEVHYRTKDVHFSAYTGATIRFGQFLSTSLLKEEAQEFGNQTLFTIFTCLGAPVQYFSLKKEVLIPPYELFKVINMSSHPRGNWLQLRSTGNLSTYNCQLLK

分子量

Approximately 40-53 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ART4/CD297 Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ART4/CD297 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P77298
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