ASAH2 蛋白, Mouse (HEK293, His)

ASAH2 protein has N-acylsphingosine amide hydrolase activity, which is essential for lipid digestion and sphingolipid metabolism. It is present in different cellular compartments, highlighting its versatility. ASAH2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ASAH2 protein, expressed by HEK293 , with N-His labeled tag.

ASAH2 蛋白因其在 N-酰基鞘氨醇酰胺水解酶活性中的作用而得到认可，而 N-酰基鞘氨醇酰胺水解酶活性是脂质消化和鞘脂代谢过程中的关键功能。它存在于不同的细胞区室中，包括小凹、细胞外空间和线粒体，强调了它的多功能性。作为质膜的一个组成部分，ASAH2 在细胞结构和功能中具有重要意义。与多个人类基因（包括 ASAH2B（N-酰基鞘氨醇酰胺水解酶 2B））的直系同源关系进一步强调了其进化保守性。该表达谱揭示了跨组织的广泛存在，在小肠成体和大肠成体中具有显着水平，表明其在维持各种生理环境中的脂质稳态和代谢过程中发挥着关键作用。

Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is 18415.400 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

N-His

Q9JHE3/NP_061300.1 (T34-T756)

54447  [NCBI]

TSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

105-115 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.