ATG5 蛋白, Human

ATG5 is an important participant in the formation of autophagy vesicles and binds to ATG12 through a ubiquitin-like system, which is critical for the lipidation of ATG8 family proteins. It plays a crucial role in mitochondrial quality control, affects cell longevity, and is critical for lymphocyte development, antigen processing, axonal morphology maintenance, adipocyte differentiation, primary ciliogenesis, and apoptotic processes. ATG5 Protein, Human is the recombinant human-derived ATG5 protein, expressed by E. coli , with tag free. The total length of ATG5 Protein, Human is 275 a.a., with molecular weight of ~33.0 kDa.

ATG5 是自噬囊泡形成的关键参与者，通过泛素样系统与 ATG12 结合，其中 ATG7 作为 E1 样激活酶，ATG10 作为 E2 样结合酶。由此产生的 ATG12-ATG5 缀合物充当 E3 样酶，对于 ATG8 家族蛋白的脂化及其与囊泡膜的关联至关重要。除了在自噬中的作用之外，ATG5 还有助于氧化损伤后的线粒体质量控制，从而影响细胞寿命。它在淋巴细胞发育中发挥着关键作用，对于 B 和 T 淋巴细胞的生存和增殖至关重要，并且对于 MHC II 的最佳抗原加工和呈递是必需的。此外，ATG5 还参与维持轴突形态、正常脂肪细胞分化，并通过自噬途径促进初级纤毛发生。此外，它可能在细胞凋亡过程中发挥关键作用，发生在 caspase 活性下游，并通过与 FADD 相互作用参与 IFN-γ 诱导的自噬细胞死亡。

Human

E. coli

Tag Free

Q9H1Y0 (M1-D275)

9474  [NCBI]

MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD

Approximately 33.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris, 0.2 M NaCl,1 mM DTT, 40%glycerol, pH 8.0 .

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Shida Yousefi, et al. Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. Nat Cell Biol. 2006 Oct;8(10):1124-32.  [Content Brief]