ATP1B1 蛋白, Mouse (Cell-Free, His)

The ATP1B1 protein is a noncatalytic component that forms a heterodimer with the α subunit and catalyzes ATP hydrolysis and Na(+) and K(+) ion exchange across the plasma membrane. These heterodimers critically regulate sodium pumping at the plasma membrane, affecting ion balance. ATP1B1 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived ATP1B1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ATP1B1 Protein, Mouse (Cell-Free, His) is 304 a.a., with predicted molecular weight of 36.7 kDa.

ATP1B1 蛋白作为活性酶的非催化成分，负责催化 ATP 水解，并伴随 Na(+) 和 K(+) 离子跨质膜的交换。作为一个重要的亚基，ATP1B1 与 α 亚基结合形成异二聚体，调节转运到质膜的钠泵的数量。除了在离子运输中的作用之外，ATP1B1 还参与重要的细胞过程，包括细胞粘附和上皮细胞极性的建立。这种多方面的功能强调了 ATP1B1 在细胞稳态、离子平衡和维持组织内结构完整性中的重要性。

Mouse

E. coli Cell-free

N-10*His

P14094 (M1-S304)

11931

MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLTISELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS

Monomer: 35 kDa Dimer: 70 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.