ATP5F1A 蛋白, Human (His)

ATP5F1A is an important component of mitochondrial ATP synthase (complex V) that coordinates ATP production from ADP by utilizing the transmembrane proton gradient. As part of the F-type ATPase, the α and β subunits of ATP5F1A form the catalytic core to achieve ATP hydrolysis. ATP5F1A Protein, Human (His) is the recombinant human-derived ATP5F1A protein, expressed by E. coli , with N-6*His labeled tag. The total length of ATP5F1A Protein, Human (His) is 510 a.a., with molecular weight of ~59.2 kDa.

ATP5F1A 是线粒体膜 ATP 合酶（F(1)F(0) ATP 合酶或复合体 V）的重要组成部分，通过利用呼吸链电子产生的跨膜质子梯度，在 ADP 生成 ATP 中发挥着关键作用交通综合体。F 型 ATP 酶由催化核心 (F(1)) 和膜质子通道 (F(0)) 组成，如 ATP5F1A，由多个具有不同功能的亚基组成。α 和 β 亚基形成 F(1) 中的催化核心，其中中心茎相对于周围 α(3)β(3) 亚基的旋转促进 ATP 水解。有趣的是，ATP5F1A 与细菌铁载体肠杆菌素结合，促进肠杆菌素衍生的铁离子在线粒体内积累。此外，ATP5F1A 与各种蛋白质相互作用，例如 ATPAF2、HRG、PLG、BLOC1S1、BCL2L1 同工型 BCL-X(L)、CLN5、PPT1、S100A1 和 ABCB7，影响从代谢效率和铁稳态到凋亡调节等多种细胞过程。此外，ATP5F1A 是 ATP 合酶复合物的重要组成部分，凸显了其在细胞能量生产中的核心作用。了解这些复杂的相互作用有助于了解 ATP5F1A 在细胞生理学中的多方面功能。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P25705 (Q44-A553)

498  [NCBI]

QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA

Approximately 59.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl，1 mM EDTA，6% Trehalose，pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.