ATP5F1B 蛋白, Human (P.pastoris, His)

ATP5F1B protein is an important component of mitochondrial membrane ATP synthase (complex V). ATP5F1B Protein, Human (P.pastoris, His) is the recombinant human-derived ATP5F1B protein, expressed by P. pastoris , with N-His labeled tag. The total length of ATP5F1B Protein, Human (P.pastoris, His) is 482 a.a., with molecular weight of ~53.8 kDa.

ATP5F1B 蛋白是线粒体膜 ATP 合酶（也称为复合体 V）的组成部分，负责在呼吸链电子传递复合物产生的跨膜质子梯度存在的情况下从 ADP 生成 ATP。F 型 ATP 酶由两个结构域组成：F(1)，包含膜外催化核心；F(0)，包含膜质子通道。这些域通过中心茎和外围茎互连。在催化过程中，F(1) 结构域中的 ATP 合成通过中心茎亚基的旋转机制与质子易位耦合。F(1) 中的催化核心由 α 和 β 亚基形成，其中中心茎相对于周围 α(3)β(3) 亚基的旋转导致 ATP 在 β 亚基上的三个独立催化位点水解。ATP5F1B 在这一复杂的过程中发挥着至关重要的作用，有助于线粒体膜内 ATP 的有效合成。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-His

P06576 (48A-529S)

506  [NCBI]

AQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

Approximately 53.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.