B2M/Beta-2-microglobulin 蛋白, Human (His)

B2M/Beta-2-microglobulin Protein is a secreted protein found on the outside of the cell membrane. B2M/Beta-2-microglobulin Protein is a crucial component of MHC class I molecules, essential for presenting tumor antigens to T cells. B2M/Beta-2-microglobulin Protein is a systemic pro-aging factor that can impair cognitive function and neurogenesis. B2M/Beta-2-microglobulin Protein, Human (His) is a recombinant human Beta-2-microglobulin protein with a His tag, expressed in E. coli^[1][2].

B2M/Beta-2-microglobulin Protein 是一种低分子量蛋白质，与免疫球蛋白具有序列同源性，存在于几乎所有有核细胞和大多数生物体液中，包括血清、尿液和滑液^[2]。
B2M/Beta-2-microglobulin Protein 与小鼠蛋白显示出 70% 的氨基酸序列相似性，两者都位于同源染色体上。该蛋白的二级结构由七条 β 链组成，它们由单个二硫键连接成两个 β 片，呈现出典型的免疫球蛋白结构域的经典 β 三明治结构^[3]。
B2M/Beta-2-microglobulin Protein 在许多细胞中以恒定水平表达，可诱导多种细胞类型中 IL-6、IL-8 和 IL-10 的表达，调节激素/生长因子的表达，协调细胞因子与其受体之间的相互作用^[2]。
B2M/Beta-2-microglobulin Protein 可以与 MHC I 或类似分子相互作用并稳定其三级结构，并广泛参与癌细胞存活、增殖、凋亡甚至转移的功能调控^[2]。
B2M/Beta-2-microglobulin Protein 与急慢性炎症、肝/肾功能不全、病毒感染和肿瘤等疾病密切相关^[2]。

B2M/Beta-2-microglobulin Protein 在 B2M 基因转染黑色素瘤细胞 FO-1 中的表达可以诱导 HLA I 类抗原表达，从而导致肿瘤细胞对自然杀伤细胞介导的裂解的敏感性降低^[4]。
靶向破坏 B2M/Beta-2-microglobulin 基因可以降低人类胚胎干细胞的免疫原性^[5]。

B2M 基因敲除小鼠中 B2M/Beta-2-microglobulin Protein 表达缺乏，会导致小鼠交配频率降低和受损的繁殖表型^[1]。
B2M 基因功能性失活小鼠中 B2M/Beta-2-microglobulin Protein 表达缺乏，会导致小鼠对糖尿病和胰岛炎具有抵抗力^[2]。
B2M/Beta-2-microglobulin Protein (100 μg/kg; 12 天内 5 次; 眶内注射) 会损伤年轻小鼠的学习记忆能力^[1]。

Human

E. coli

N-6*His

P61769 (I21-M119)

567  [NCBI]

IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Approximately 14.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, PH 7.4 or PBS, 300 mM NaCl, pH 7 or 20 mM PB, 300 mM NaCl, pH 7.4, 10% trehalose and 0.02% Tween80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Smith LK, et al. β2-microglobulin is a systemic pro-aging factor that impairs cognitive function and neurogenesis. Nat Med. 2015 Aug;21(8):932-7.  [Content Brief]

  [2]. Li L, et al. The Implication and Significance of Beta 2 Microglobulin: A Conservative Multifunctional Regulator. Chin Med J (Engl). 2016 Feb 20;129(4):448-55.  [Content Brief]

  [3]. Saper MA, et al. Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution. J Mol Biol. 1991 May 20;219(2):277-319.  [Content Brief]

  [4]. Maio M, et al. Reduction in susceptibility to natural killer cell-mediated lysis of human FO-1 melanoma cells after induction of HLA class I antigen expression by transfection with B2m gene. J Clin Invest. 1991 Jul;88(1):282-9.  [Content Brief]

  [5]. Wang D, et al. Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. Stem Cells Transl Med. 2015 Oct;4(10):1234-45.  [Content Brief]

  [6]. Cooper JC, et al. An impaired breeding phenotype in mice with a genetic deletion of beta-2 microglobulin and diminished MHC class I expression: role in reproductive fitness. Biol Reprod. 2007 Aug;77(2):274-9.  [Content Brief]

  [7]. Serreze DV, et al. Major histocompatibility complex class I-deficient NOD-B2mnull mice are diabetes and insulitis resistant. Diabetes. 1994 Mar;43(3):505-9.  [Content Brief]