B7-2/CD86 蛋白, Human (HEK293, Fc)

B7-2/CD86 Protein, Human (HEK293, Fc) is a polypeptide chain with the C-termimal human IgG1 Fc fragment produced in HEK293 cells. B7-2 (CD86) is a costimulatory molecule expressed by antigen-presenting cells (APCs), which interacts with CD28 for T-cell activation and survival and with CTLA4 for immune regulation.

CD86 是一个跨膜区域，并且具有比 CD80 更长的细胞质结构域。CD86 在叉指树突状细胞 (DC)、朗格汉斯细胞、外周血 DC、记忆 B 细胞和生发中心 B 细胞以及巨噬细胞上组成型表达。通过 Ig 受体交联或添加多种细胞因子激活后，B 细胞上的 CD86 迅速上调^[1]。

Immobilized B7-2/CD86-Fc at 5 μg/mL (100 μL/well) can bind human Biotin-CD28-Fc with a linear range of 0.09~3.12μg/mL.

Human

HEK293

C-hFc

P42081 (L20-P247)

942  [NCBI]

LSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP

65-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Han L, et al. A monoclonal antibody against CD86 and its protection in a murine lupus nephritis model of chronic graft-versus-host disease. Immunopharmacol Immunotoxicol. 2017 Oct;39(5):285-291.  [Content Brief]