B7-2/CD86 蛋白, Rabbit (HEK293, His)

The B7-2/CD86 protein is a key receptor that provides costimulatory signals critical for T lymphocyte proliferation and interleukin-2 production through CD28 or CTLA-4 binding. It plays a decisive role in early T cell activation, affecting immunity or anergy within 24 hours. B7-2/CD86 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived B7-2/CD86 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of B7-2/CD86 Protein, Rabbit (HEK293, His) is 224 a.a., with molecular weight of 39-67 kDa.

B7-2/CD86 蛋白作为 T 淋巴细胞增殖和白细胞介素 2 生成所需的共刺激信号的关键受体发挥作用，通过与 CD28 或 CTLA-4 结合来实现。它在 T 细胞激活和初始 T 细胞共刺激的早期事件中发挥着至关重要的作用，有助于在激活后 24 小时内做出免疫和无反应的决定。此外，B7-2/CD86 参与 B 细胞功能的调节，影响产生的 IgG(1) 水平。CD40 参与后，它通过磷脂酶 C 和蛋白激酶 C 激活来激活 NF-kappa-B 信号通路。该蛋白形成同二聚体并与 MARCH8 相互作用。此外，它与 PHB1 和 PHB2 结合，用 CD40 引发后相互作用增强。

Rabbit

HEK293

C-6*His

P42071 (A23-R246)

100008999  [NCBI]

AASLRIQAYFNKTADLPCQFTNSQSRSLSELVVFWQDQERLVLYELFLGREKPDNVDPKYIGRTSFDQESWNLQLHNVQIKDKGVYQCFVHHRGAKGLVPIYQMNSELSVLANFTQPEITLISNITRNSAINLTCSSVQGYPEPKKMFFVLKTENATTEYDGVIEKSQDNVTGLYNISISGSITFSDDIRNATIYCVLQTESTETYSQHFPIVPADPVPVEKPR

39-67 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.