1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. BCAT2 蛋白, Human

BCAT2 蛋白, Human

目录号: HY-P701339
产品使用指南

各种研究表明,BCAT2 蛋白在细胞过程中发挥着关键作用,因为它催化必需支链氨基酸亮氨酸、异亮氨酸和缬氨酸分解代谢的初始反应。这种酶活性对于这些氨基酸的分解至关重要,有助于能量产生和氮平衡所需的代谢过程。BCAT2 蛋白, Human 是重组的 BCAT2 蛋白,由 E. coli 表达,不带标签。BCAT2 蛋白, Human 全长 365 个氨基酸。

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描述

Various studies have demonstrated that the BCAT2 protein plays a critical role in cellular processes as it catalyzes the initial reactions in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine. This enzyme activity is essential for the breakdown of these amino acids, contributing to metabolic processes necessary for energy production and nitrogen balance. BCAT2 Protein, Human is the recombinant human-derived BCAT2 protein, expressed by E. coli , with tag free. The total length of BCAT2 Protein, Human is 365 a.a., .

研究背景

BCAT2(支链氨基酸转氨酶 2)在必需支链氨基酸亮氨酸、异亮氨酸和缬氨酸的分解代谢中发挥着关键作用。它催化该过程中的第一个反应,涉及这些氨基酸可逆地转氨为各自的支链 α-酮酸。这种酶活性对于调节支链氨基酸及其代谢物的水平至关重要,它们参与各种生理过程,包括能量代谢和蛋白质合成。此外,BCAT2 可以充当支链 α-酮酸的转运蛋白,有助于其细胞转运。参考文献简洁地概述了 BCAT2 在支链氨基酸分解代谢中的核心作用,并表明其可能参与相关代谢物的细胞内转运。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

O15382 (A28-V392)

基因 ID

587

蛋白结构
N-term
BCAT2 (A28-V392)
Accession # O15382
C-term
同用名
BCAT2; Branched-chain-amino-acid aminotransferase; mitochondrial; BCAT(m); Placental protein 18; PP18
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BCAT2 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BCAT2 Protein, Human
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HY-P701339
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