BD-3 蛋白, Mouse

BD-3 Protein, Mouse is an inducible antimicrobial peptide and exhibits broad-spectrum antimicrobial activity.

由杆状病毒表达系统产生的重组小鼠 Beta Defensin-3 肽显示出针对幽门螺杆菌的抗菌活性。铜绿假单胞菌 PAO1（MIC 为 8 μg/mL）和大肠杆菌 D31（MIC 为 16 μg/mL）以盐依赖性方式^[1]。小鼠 Beta Defensin-3 肽具有广谱抗菌活性，该肽可作为小鼠特定粘膜表面针对微生物入侵的先天防御^[2]。

Measured by its anti-microbial activity against E. coli. The ED₅₀ this effect is 387.3 ng/mL, corresponding to a specific activity is 2.58×10^3 U/mg.

• 
  Measured by its anti-microbial activity against E. coli. The ED₅₀ for this effect is 387.3 ng/mL, corresponding to a specific activity is 2.58×10³ U/mg.

Mouse

E. coli

Tag Free

Q9WTL0 (K23-K63)

27358  [NCBI]

KKINNPVSCLRKGGRCWNRCIGNTRQIGSCGVPFLKCCKRK

Approximately 10.06 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 40 mM PB, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bals R, et al. Mouse beta-defensin 3 is an inducible antimicrobial peptide expressed in the epithelia of multiple organs. Infect Immun. 1999 Jul;67(7):3542-7.  [Content Brief]

  [2]. Burd RS, et al. Murine beta-defensin-3 is an inducible peptide with limited tissue expression and broad-spectrum antimicrobial activity. Shock. 2002 Nov;18(5):461-4.  [Content Brief]