1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Beta-galactosidase/GLB1 蛋白, E.coli

Beta-galactosidase/GLB1 蛋白, E.coli

目录号: HY-P7859
COA 产品使用指南

Beta-galactosidase/GLB1 蛋白是一种水解酶,具有催化活性及 Mg2+、Mn2+ 和 Na+ 结合位点。Beta-galactosidase/GLB1 蛋白可用于遗传学、分子生物学和其他生命科学的研究。Beta-galactosidase/GLB1 蛋白, E.coli 是重组的 β-半乳糖苷酶/GLB1 蛋白,由 E. coli 表达,不带标签。

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规格 价格 是否有货 数量
10 μg ¥700 In-stock
50 μg ¥1900 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Beta-galactosidase/GLB1 protein is a hydrolase with catalytic activity and binding sites of Mg2+, Mn2+ and Na+. Beta-galactosidase/GLB1 protein can be used for research in genetics, molecular biology, and other life sciences. Beta-galactosidase/GLB1 Protein, E.coli is the recombinant E. coli-derived Beta-galactosidase/GLB1 protein, expressed by E. coli , with tag free.

研究背景

Beta-galactosidase 是一种广泛存在于自然界植物中可由大肠杆菌产生的水解酶。Beta-galactosidase由四条相同的、异常长的多肽链组成的四聚体,有 9-10 个亚基组成,氨基酸组成和大肠杆菌酶有很大不同。Beta-galactosidase 可与Mg2+、Mn2+ 和Na+ 结合。Beta-galactosidase 可被苯乙基硫代-β-D-半乳糖(PETG)、异丙基硫代-β-D-半乳糖(IPTG)、L-核糖、D-半乳糖内酯、乳糖和2-氨基-D-半乳糖抑制。Beta-galactosidase 具有较大的分子量,易于分离纯化。Beta-galactosidase 可用于食品方面的研究。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

E.coli

表达系统

E. coli

标签

Tag Free

蛋白编号

P00722 (M1-K1024, M443L, C500S, 12-41a.a deletion)

基因 ID

945006  [NCBI]

蛋白结构
N-term
Beta-gal (M1-K1024, M443L, C500S, 12-41a.a deletion)
Accession # P00722
C-term
同用名
rEcoBeta-galactosidase; Beta-galactosidase; Beta-gal; Lactase; lacZ
氨基酸序列

MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK

分子量

Approximately 115.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl,150 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Beta-galactosidase/GLB1 Protein, E.coli 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Beta-galactosidase/GLB1 Protein, E.coli
目录号:
HY-P7859
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