2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Beta-galactosidase 蛋白, Saccharolobus solfataricus (FLAG, His)

Beta-galactosidase 蛋白, Saccharolobus solfataricus (FLAG, His)

目录号: HY-P701892
产品使用指南

β-半乳糖苷酶蛋白具有催化活性，水解β-D-半乳糖苷中末端非还原性β-D-半乳糖残基。其酶功能包括破坏这些特定的键，促进 β-D-半乳糖苷的裂解，并有助于含有这些末端半乳糖残基的分子的降解。Beta-galactosidase 蛋白, Saccharolobus solfataricus (FLAG, His) 是重组的 β-半乳糖苷酶蛋白，由 E. coli 表达，带有 N-6*His, N-Flag 标签。Beta-galactosidase 蛋白, Saccharolobus solfataricus (FLAG, His) 全长 489 个氨基酸。

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描述

Beta-galactosidase Protein, with catalytic activity, hydrolyzes terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Its enzymatic function involves breaking these specific bonds, facilitating the cleavage of beta-D-galactosides and contributing to the degradation of molecules containing these terminal galactose residues. Beta-galactosidase Protein, Saccharolobus solfataricus (FLAG, His) is the recombinant Beta-galactosidase protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of Beta-galactosidase Protein, Saccharolobus solfataricus (FLAG, His) is 489 a.a., .
研究背景

β-半乳糖苷酶蛋白具有催化活性，特别参与β-D-半乳糖苷中末端非还原性β-D-半乳糖残基的水解。其酶功能涉及破坏这些特定的化学键，促进 β-D-半乳糖苷的裂解，并有助于含有这些末端半乳糖残基的分子的降解。
种属

Others
表达系统

E. coli
标签

N-6*His;N-Flag
蛋白编号

P22498 (M1-H489)
基因 ID

72911572
蛋白结构
N-term
Flag-6*His
Beta-galactosidase (M1-H489)
Accession # P22498
C-term
同用名
lacS; Beta-galactosidase; Lactase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Beta-galactosidase Protein, Saccharolobus solfataricus (FLAG, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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